American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

How should a new batch of hypotonic solution be evaluated for successful harvest?

1. Testing for pH levels

2. Comparing to a lot already in use

3. By visual inspection

4. Performing a viability test

The correct answer is: Comparing to a lot already in use

Evaluating a new batch of hypotonic solution is essential to ensure that it functions effectively during the cytogenetic harvest process. The correct approach is to compare the new batch to a lot already in use that has shown successful results. This comparison provides a benchmark based on established performance, allowing for assessment of factors such as osmotic strength and effectiveness in cell lysis. This method ensures that the new batch is consistent with the quality and characteristics of the previously validated solution, which is critical for achieving optimal cell harvest and chromosome visualization. If any significant differences are noted in the performance, the new batch may not be suitable for use. While testing for pH levels, visual inspection, and performing a viability test are indeed important quality control measures in laboratory practices, they do not directly relate to confirming the effectiveness of a hypotonic solution for harvesting cells. pH testing is crucial but does not provide a comprehensive assessment of hypotonic solution function. Visual inspection may indicate clarity or particulate matter but lacks quantitative data on effectiveness. A viability test assesses cell health rather than the performance of the hypotonic solution in lysis. Thus, utilizing the established lot as a reference remains the most relevant and effective evaluation strategy.