American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

How should a new batch of hypotonic solution be evaluated for successful harvest?

1. By testing it on a control sample

2. By comparing it to the old solution in use

3. By consulting with a supervisor

4. By observing the color change

The correct answer is: By comparing it to the old solution in use

Evaluating a new batch of hypotonic solution for successful harvest is crucial in cytogenetics procedures. The correct approach is to test it against an established parameter, which often means comparing it directly to the old solution in use. This comparison allows you to assess whether the new batch behaves similarly in functionality and effectiveness. Hypotonic solutions are used to lyse cells and facilitate the spreading of chromosomes for microscopic examination. If the new solution fails to yield results comparable to the old one, it could indicate a problem with its composition or effectiveness. Observing the characteristics and effects of the new solution side-by-side with the previous one ensures reliability in laboratory practices. While testing the new solution on a control sample could provide some information, it does not directly assess the solution's effectiveness in comparison to a known standard. Consulting with a supervisor is certainly valuable, but it does not provide immediate empirical evidence regarding the performance of the new batch. Observing color changes may not be a reliable metric for determining the efficacy or quality of the hypotonic solution, as it may not guarantee a functional assessment.