American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

In relation to microscope objectives, what does NA stand for?

1. Numerical Aperture

2. New Aperture

3. Necessary Adjustment

4. Neutral Angle

The correct answer is: Numerical Aperture

NA stands for Numerical Aperture, which is a crucial concept in microscopy that quantifies the ability of a lens to gather light and resolve fine specimen detail at a fixed object distance. It is a dimensionless number that is a function of the refractive index of the medium in which the lens is working and the sine of the half-angle of the maximum cone of light that can enter or exit the lens. A higher numerical aperture indicates a greater ability of the lens to gather light, resulting in better resolution and brighter images. This characteristic is essential for cytogenetics, where clear and precise imaging is necessary for analyzing cellular structures and chromosomal arrangements. Understanding numerical aperture helps in selecting the appropriate objective lenses for specific applications in microscopy, ultimately enhancing the quality of the observations and analyses.