American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

The use of filters impacts which aspect of microscope operation?

1. Focus quality

2. Contrast of images

3. Light transition speed

4. Objective lens placement

The correct answer is: Contrast of images

The impact of filters on microscope operation primarily relates to the contrast of images. Filters are used in microscopy to selectively block or transmit specific wavelengths of light. By doing so, they can enhance the contrast between different components of the specimen being observed. For example, a red filter can be utilized to improve the visibility of certain cellular structures against a background that otherwise would not be distinct without the filter. Using filters can also aid in visualizing fluorescent samples, where specific wavelengths are necessary to excite dyes or fluorescent proteins. Thus, the appropriate filter can significantly improve the clarity and detail in the resulting images, contributing to better interpretation of the sample. The other aspects mentioned, such as focus quality, light transition speed, or objective lens placement, are not directly influenced by the use of filters. Focus quality mainly depends on the alignment and quality of the optics, light transition speed is more linked to the microscope's light source and settings, and objective lens placement refers to the physical positioning of the lens relative to the specimen. Therefore, the correct choice focuses on how filters enhance image contrast, thereby improving the overall microscopy experience.