American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

What could contaminate amniotic fluid samples if proper precautions are not taken?

1. Staphylococcus

2. Mycoplasma

3. E. coli

4. Streptococcus

The correct answer is: Mycoplasma

In the context of amniotic fluid sampling, Mycoplasma is a particularly critical concern because it represents a unique challenge due to its small size and lack of a cell wall, making it more resilient in various environments. Mycoplasma species are often present in the genital tracts and can easily contaminate samples if aseptic techniques are not strictly followed. The ability of Mycoplasma to exist in non-culturable forms also complicates detection, which can lead to misinterpretation of the results, impacting patient management and outcomes. To emphasize the importance of adhering to stringent aseptic techniques during the collection and handling of amniotic fluid, it’s essential to recognize that while other bacteria like Staphylococcus, E. coli, and Streptococcus can also be contaminants, the unique characteristics of Mycoplasma make it especially concerning in the context of cytogenetic testing. Proper equipment sterilization, handling protocols, and environmental control are critical in preventing Mycoplasma contamination.