American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

What impact does a higher pH have on cell cultures?

1. Encourages cell growth

2. Inhibits cell growth

3. Has no effect on cell metabolism

4. Stimulates apoptosis

The correct answer is: Inhibits cell growth

A higher pH in cell cultures can lead to conditions that are not optimal for most cell types, which can inhibit cell growth. In many mammalian cell cultures, the ideal pH range is typically around 7.2 to 7.4. When the pH rises significantly above this range, it can disrupt cellular processes by affecting enzyme activity, metabolism, and overall cellular function. This disruption often leads to a decline in cell viability and proliferation, which is why inhibiting cell growth is the result associated with higher pH levels. While some certain bacterial or fungal cultures might thrive at higher pH levels, the majority of mammalian cells exhibit decreased growth rates and altered metabolic activity under alkaline conditions. This adverse impact is particularly critical in various research and therapeutic applications where precise growth conditions are necessary for successful outcomes.