American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

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What is the method for adjusting the brightness of a microscope?

  1. Adjust the regulating transformer or add filters

  2. Rotate the eyepiece to increase luminosity

  3. Change the objective lens to a higher power

  4. Use the stage controls to elevate the specimen

The correct answer is: Adjust the regulating transformer or add filters

The method for adjusting the brightness of a microscope involves utilizing a regulating transformer or adding filters to control the intensity of light passing through the specimen. This is essential for achieving optimal visibility of the sample without causing optical distortion or glare. Regulating transformers allow for precise control of light levels, enabling adjustments based on the specific requirements of different specimens and observation techniques. Filters can be used to alter the spectrum or intensity of light, enhancing contrast and providing a clearer view of the cellular components being studied. The other options do not effectively address brightness adjustments. Rotating the eyepiece would not influence light intensity, as it is primarily for viewing comfort and not illumination control. Changing to a higher power objective lens typically decreases the amount of light reaching the eyepiece, making it less effective for brightness adjustment. Elevating the specimen with stage controls affects the focus and positioning but does not impact the brightness of the light that illuminates the object.