American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

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What is the purpose of the light histogram in an automated karyotype system?

  1. For capturing video footage

  2. To achieve optimal light intensity and enhance image quality

  3. To reduce the amount of light used

  4. For measuring the specimen dimensions

The correct answer is: To achieve optimal light intensity and enhance image quality

The correct choice highlights the role of the light histogram in optimizing the overall performance of an automated karyotype system. The light histogram is a graphical representation that helps in analyzing the distribution and intensity of light captured from samples during imaging. By achieving optimal light intensity, the system enhances the quality of images produced, which is crucial for accurate analysis of chromosomal structures and abnormalities. This improvement in image quality leads to better identification and delineation of chromosomes, facilitating more reliable and precise karyotyping. Other options may involve aspects of imaging but do not directly relate to the primary function of the light histogram in an automated karyotype system. For example, capturing video footage or measuring specimen dimensions, while potentially useful in a research or clinical setting, do not pertain to the specific purpose of the light histogram, which is focused on light intensity and image enhancement. Likewise, while reducing the amount of light can have some advantages, it does not align with the primary goal of achieving optimal image quality, which is essential for accurate cytogenetic interpretation.