American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

What is the significance of using SKY probes in metaphase analysis?

1. They provide a higher resolution

2. They allow for simultaneous detection of multiple targets

3. They enhance the contrast of the sample

4. They reduce the background noise

The correct answer is: They allow for simultaneous detection of multiple targets

Using SKY (Spectral Karyotyping) probes in metaphase analysis is significant because they allow for simultaneous detection of multiple targets. This capability stems from the unique labeling of each chromosome with different fluorescent dyes, enabling the identification of all 24 different chromosomes in a single hybridization step. The spectral imaging technology makes it possible to distinguish overlapping emission spectra from various probes, leading to a more comprehensive analysis of chromosomal abnormalities. This simultaneous detection is particularly useful in complex cases of aneuploidy or structural chromosome rearrangements, as it saves time and reduces the potential for human error associated with analyzing chromosomes one at a time. The ability to visualize multiple targets at once also aids in providing a more complete picture of the chromosomal landscape in a given sample, which is critical for accurate diagnosis and subsequent clinical decision-making. Other options may address different aspects of cytogenetic analysis, such as resolution, contrast, or background noise reduction, but they do not encapsulate the primary advantage of SKY, which is its ability to facilitate the concurrent identification of several chromosomal targets. This feature revolutionizes traditional karyotyping methods and enhances the overall efficiency and effectiveness of cytogenetic evaluations.