American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

What is typically included in the specifications of a PlanApo lens?

1. A 50/1.0 numerical aperture

2. 100x magnification and 1.4 NA

3. 40x magnification with a 0.17 cover slip

4. A 160mm tube length

The correct answer is: 100x magnification and 1.4 NA

The specifications for a PlanApo lens typically include 100x magnification and a numerical aperture (NA) of 1.4. This combination is common for high-resolution imaging in microscopy, particularly in applications involving cytogenetics and cellular biology. The 100x magnification allows for detailed observation of cellular structures, which is critical when analyzing chromosomal abnormalities or other fine details within cells. The numerical aperture of 1.4 is significant because it indicates the lens's ability to gather light and resolve fine specimen detail at a high magnification level. A higher NA increases the lens's resolution, making it essential for cytogenetic analysis where clarity and detail are paramount. In contrast, while other magnification and NA values could be relevant in different contexts, they do not represent the typical specifications for a PlanApo lens. For instance, a 50/1.0 numerical aperture or a 40x magnification with a different NA may not provide the same level of resolution and detail necessary for accurate microscopy work. Similarly, the mention of a 160mm tube length relates to the optical setup and compatibility with certain types of microscopes but is not specific to the PlanApo lens's magnification and resolution capabilities.