American Society for Clinical Pathology (ASCP) Technologist in Cytogenetics certification (ASCP CG) Practice Test

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Why is it necessary to adjust the light amount after changing the objective lens?

To improve the overall clarity
Higher magnification requires more light
To avoid light burn
It ensures the lens does not break

The correct answer is: Higher magnification requires more light

When changing the objective lens on a microscope, it is essential to adjust the light amount because higher magnification lenses typically require more light to produce a clear and detailed image of the specimen. As the magnification increases, the diameter of the lens opening decreases, which can limit the amount of light that enters. Therefore, increasing the light intensity compensates for this limitation, allowing for a better visual quality and detail that is crucial in cytogenetics analysis. Proper light adjustment ensures optimal visibility of cellular structures, which is vital for accurate observations and interpretations in cytogenetic studies.